Structural biology of membrane proteins is a major focus of our research. Membrane proteins mediate key life processes: cells employ membrane proteins to harness energy, sense the environment, and import molecules. Understanding the mechanisms of these critical processes, as well as designing pharmaceuticals targeting membrane proteins to treat diseases, is limited by the challenging nature of membrane protein studies. Recent advances in membrane protein expression and structure determination make this an exciting time to work on membrane proteins and advance this frontier area in biology. Our laboratory combines a variety of tools including solid-state NMR and mass spectrometry with other biophysical, biochemical, and molecular biology approaches to address structure and function in important membrane proteins. We study bacterial chemotaxis receptors to understand mechanisms of transmembrane signaling and ABC transporters to understand mechanisms of transmembrane transport.