University of Massachusetts
Department of Chemistry
 
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 © 2007
University of Massachusetts Amherst


 
 

Literature

 
Caspase Papers
  Judy Ventura, Scott J. Eron, Daniella C. Gonzalez-Toro, Kishore Raghupathi, Feng Wang, Jeanne A Hardy*, and S. Thayumanavan* (2015) Reactive Self-Assembly of Polymers and Proteins to Reversibly Silence a Killer Protein. Biomacromolecues. DOI: 10.1021/acs.biomac.5b00779  
  Mout R, Zhao Y, Yeh YC, Tang R, Jeong Y, Duncan B, Hardy JA, Rotello VM. (2015) Co-Delivery of Protein and Small Molecule Therapeutics Using Nanoparticle-Stabilized Nanocapsules. Bioconjug Chem. 26 (5), 950–954.  
  Dagbay. K.B., Eron. S.J., Serrano, B.P., Zhao, Y., Velázquez-Delgado, E.M., Lin, D. and Hardy, J.A. (2014). A multi-pronged approach for compiling a global map of allosteric regulation in the apoptotic caspases. Methods in Enzymology, UK: Academic Press, 544: 215-249.  
  Tang R, Kim CS, Solfiell DJ, Rana S, Mout R, Velázquez-Delgado EM, Chompoosor A, Jeong Y, Yan B, ZhuZJ, Kim C, Hardy JA, Rotello VM. (2013) Direct delivery of functional proteins and enzymes to the cytosol using nanoparticle-stabilized nanocapsules. ACS Nano. 7(8):6667-73.  
 

Velazquez-Delgado, E. M. and Hardy, J. A., 2012. “Zinc-Mediated Allosteric Inhibition of Caspase-6.”Journal of Biological Chemistry. 287(43), 36000.

pdf
  Huber, K.L. and Hardy J.A., 2012 “Mechanism of zinc-mediated inhibition of caspase-9.” Protein Science. 21,1056-1065.. Article featured as cover illustration. pdf
  Huber, K.L., Ghosh, S. and Hardy J.A., 2012. “Inhibition of caspase-9 by stabilized peptides targeting the dimerization interface.”Peptide Science. 98(5), 451–465. pdf
  Velazquez-Delgado, E. M. and Hardy J.A., 2012. “Phosphorylation regulates assembly of the caspase-6 substrate-binding groove.” Structure. 20, 742–751.

pdf; preview; press release

  Vaidya S., Hardy J.A., Caspase-6 Latent State Stability Relies on Helical Propensity.
Biochemistry 2011, 50(16):3282-7.
pdf
  Vaidya S., Velázquez-Delgado E.M., Abbruzzese G., Hardy J.A. Substrate-Induced Conformational Changes Occur in All Cleaved Forms of Caspase-6. Journal of Molecular Biology 2011, 406(1):75-91. pdf
  Witkowski, W.A., Hardy, J.A. "L2 loop is critical for caspase-7 active site formation" Protein Science, 2009, 18, 1459-1468. Article featured as cover illustration.

pdf

 
Witold's movie of conformational changes in the capsase mutant I213A.
 
  Hardy, J. A. and Wells, J.  “Dissecting an Allosteric Switch in Caspase-7 using Chemical and Mutational Probes.” Journal Biological Chemistry 2009, 284(38):26063-9. pdf
  Hardy, J. A., Lam, J., Nguyen, J. T., O’Brien, T. and Wells, J. “Discovery of an allosteric site in caspases.” Proc. Natl. Acad. Sci. U S A. 2004, 101(34), 12461-6. pdf
movie
     
     
     
Protein Design Papers  
  Maureen E. Hill, Derek J. MacPherson, Peng Wu, Olivier Julien, James A. Wells, Jeanne A. Hardy 2015 “Reprogramming Caspase-7 specificity to Caspase-6 by regio-specific mutations and selection” Under Revision ACS Chemical Biology

 

superposition

 

Wu, P., Nicholls, S., and Hardy, J.A. 2013 “A tunable, modular approach to fluorescent protease-activated reporters.”Biophysical Journal. 104(7):1605-14.

pdf
 

Nicholls, S. and Hardy, J.A. 2013 “Structural Basis of Fluorescence Quenching in Caspase Activatable-GFP.” Protein Science. 22(3), 247. Article featured as cover illustration.

pdf
  López, G.E, Colón-Díaz, I., Cruz, A., Ghosh,S., Nicholls, S.B., Viswanathan, U., Hardy, J.A. and Auerbach, S.M. 2012 “Modeling non-aqueous proton wires tethered to helical peptides: Biased proton transfer driven by helical dipoles.” The Journal of Physical Chemistry A. 116(4):1283-8. pdf
 

Nicholls, S., Chu, J., Abbruzzese, G. Tremblay, K.D. and Hardy, J.A. “Mechanism of a dark-to- bright reporter of caspase activity.”Journal of Biological Chemistry, 286 (28), 24977-24986.

pdf; press release

CA-GFP Movie

 

Witkowski, W. and Hardy, J.A. “A designed redox-controlled caspase”Protein Science, 20, 1421-1431. Article featured as cover illustration.

pdf
  Huber, K.L., Olson, K.D. and Hardy, J.A. "Robust production of a peptide library using methodological synchronization" Protein Expression and Purification. 2009, 67,139-147. pdf
  Hardy, J. A. and Nelson, H. C. M. “Proline in an alpha-helical kink is required for folding kinetics but not for kinked structure, function or stability of heat shock transcription factor” Protein Science, 2000, 9(11), 2128-2141. pdf
  Hardy, J. A., Walsh, S. T. R., and Nelson, H. C. M. “Role of an alpha-Helical Bulge in the Yeast Heat Shock Transcription Factor” J. Mol. Biol., 2000, 295(3), 393-409. pdf
     
     
     
Allosteric Papers  
  Hardy, J. A. and Wells, J. “Searching for Allosteric Sites in Enzymes.” Curr. Opin. Str. Biol. 2004, 14(6), 706-715. pdf
  Hardy, J.A. "Discovery and exploitation of allosteric sites for control of protein function." Nanomedicine: Nanotechnology, Biology and Medicine 2006, 2(4), 291.  
 

Hardy, J. A. “A Link Means a Lot: Disulfide Tethering in Structure-Based Drug Design” in Computational Approaches to Structure Based Drug Design, publishers Royal Society of Chemistry, 2007, 318-347.

pdf
           
Funding
  Smith Family Young Investigator Award from The Medical Foundation 2005-2007  
  Beckman Young Investigator Award from the Arnold and Mabel Beckman Foundation 2006-2010
  American Cancer Society Institutional Research Grant 2006-2007  
  NSF Chemical Bonding Center "Fueling the Future" 2007-2010  
  NIH R01 Grant 2008-2018  
  NSM Excellence Initiative Grant 2008  
  Cottrell Scolar Award from Research Corporation 2008-2012  
  Center of Excellence in Apoptosis Grant 2009-2012  
  NIH R03 Grant 2012-2014  
  Armstrong Grant 2013-2015  
         
           
           
           
           
           

 


 

Lab Updates

9-1-15
Bay Serrano wins best talk prize at ResearchFest. Kevin Dagbay and Scott Eron win poster prizes!

9-2-15
Scott Eron's paper in collaboration with the Thai lab was accepted in Biomacromolecules .7-24-15
NIH awards supplement for new Hardy Lab plate reader.

 



 

 

 

 

 



 

 
   

 

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